Assignment Name: ______________________________ CHEM 4500 Extra Credit 1. A research scientist has collected the data shown below, wherein a series of

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Assignment Name: ______________________________

CHEM 4500

Extra Credit

1. A research scientist has collected the data shown below, wherein a series of experiments were performed under conditions of varied substrate concentration and constant total enzyme concentration. Please use this data to answer the following questions. Assume a total enzyme concentration equal to 5 micromolar.

[S] (mM)

Vo (mM * s-1)

1

2.5

2

4.0

5

6.3

10

7.6

20

9.0

30

9.2

40

9.1

a. Please calculate values for the KM,Vmax, and catalytic efficiency using a double-reciprocal plot. For full credit, please provide the plot and all related calculations. (2 Points)

b. Please calculate values for the KM,Vmax, and catalytic efficiency using an Eadie-Hofstee plot. For full credit, please provide the plot and all related calculations. (2 Points)

c. Please calculate values for the KM,Vmax, and catalytic efficiency using an [S]/v versus [S] plot. For full credit, please provide the plot and all related calculations. (2 Points)

d. Please briefly discuss differences in the estimated parameters derived from each of the above plots. How similar/different are the estimated values? Why might different plotting strategies yield different parameter estimes? (2 Points)

2. Please draw representative double-reciprocal plots for competitive and uncompetitive inhibition scenarios (1 Point). Please provide a qualitative description that compares and contrasts the two plots. Please specifically address why the x- and y-intercepts are impacted (1 Point).

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